Môi trường NBRC 979

Môi trường NBRC 979
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Medium Detail Information

 

Medium No.
979
Medium
Clostridium thermocellum Medium (D58 Medium)
Composition
(NH4)2SO41.3g
MgCl2·6H2O2.6g
KH2PO41.43g
K2HPO4·3H2O7.2g
CaCl2·2H2O0.13g
Sodium glycerophosphate6g
FeSO4·7H2O1.1mg
Glutathione0.25g
Yeast extract4.5g
Resazurin1mg
Cellulose (Avicel or MN 300)10g
or Cellobiose5g
Distilled water1L
pH 7.0-7.2
Mix ingredients except for cellobiose and autoclave under N2/CO2(80/20) condition. Aseptically and anaerobically add filter-sterile 10% cellobiose solution before inoculation.
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