Môi trường JCM 19

Môi trường JCM 19
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Medium data

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19   BIFIDOBACTERIUM MEDIUM

Casein peptone, tryptic digest 10.0    g
Beef extract (BD-Difco) 5.0    g
Yeast extract (BD-Difco) 5.0    g
Glucose 10.0    g
K2HPO4 3.0    g
Tween 80 1.0    ml
Distilled water 1.0    L

Adjust pH to 6.8.
After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine·HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances.