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19 BIFIDOBACTERIUM MEDIUM
|Casein peptone, tryptic digest||10.0||g|
|Beef extract (BD-Difco)||5.0||g|
|Yeast extract (BD-Difco)||5.0||g|
Adjust pH to 6.8.
After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine·HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances.