Môi trường NBRC 3

Medium No.
Potato 200 g
Glucose 120 g
NaCl 50 g
Distilled water 1 L
Agar 20 g
pH 5.6
Wash potatoes with tap water, peel and cut into 1 cm cubes. Rinse with tap water quickly and boil 200 g of potato cubes with 1 L of distilled water for 20 min. Mash and squeeze through a muslin bag. Add agar and boil till melted. Add glucose and NaCl, and stir till dissolved. Make up to 1 L. Adjust pH. Autoclave to sterilize.