Medium data
Search for grmd=[13].
13 BL AGAR (GLUCOSE BLOOD LIVER AGAR)
| Lab-lemco powder (Oxoid) | 3.0 | g |
| Proteose peptone No. 3 (BD-Difco) | 10.0 | g |
| Trypticase peptone (BD-BBL) | 5.0 | g |
| Phytone peptone (BD-BBL) | 3.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Liver extract (see below) | 150.0 | ml |
| Glucose | 10.0 | g |
| Soluble starch | 0.5 | g |
| Solution A (see below) | 10.0 | ml |
| Solution B (see below) | 5.0 | ml |
| Tween 80 | 1.0 | g |
| Bacto agar (BD-Difco) | 15.0 | g |
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| Horse blood | 50.0 | ml |
| Distilled water | 825.0 | ml |
Adjust pH to 7.2.
To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.
Solution A:
| K2HPO4 | 10.0 | g |
| KH2PO4 | 10.0 | g |
| Distilled water | 100.0 | ml |
Solution B:
| MgSO4·7H2O | 4.0 | g |
| NaCl | 0.2 | g |
| FeSO4·7H2O | 0.2 | g |
| MnSO4·xH2O | 0.2 | g |
| Distilled water | 100.0 | ml |
Mix ingredients except horse blood and adjust pH to 7.2. After autoclaving, cool to 50C, and aseptically add 50.0 ml of horse blood.
Comment: Commercial BL agar (Nissui) is also available.
