Komagataella pastoris – NBRC 10777
|Other Culture Collection No.
|ATCC 28485=BCRC 21531=CBS 704=IAM 12903=JCM 3650=NCYC 175=NRRL Y-1603=OUT 20010
|Glass ampoule (L-dried)
|Source of Isolation
|Exudate of chestnut tree
|Locality of Source
|Country of Origin
|IFO 10777 <- Dept. Agric. Chem., Shizuoka Univ. (Y. Yamada) <- JCM 3650 <- AJ 5076 <- CBS 704
Komagataella pastoris, is a methylotrophic yeast that belongs to the class Ascomycetes.
It normally exists in the vegetative haploid state. Vegetative reproduction is by multilateral budding. Nitrogen limitation stimulates mating and leads to the formation of diploid cells.
Komagataella pastoris is considered to be homothallic because cells of the same strain can mate with each other. There may be more than one mating type in the population, which switches at a high frequency so that mating occurs between haploid cells of opposite mating type. Diploid cells maintained in standard vegetative growth medium remain diploid. If they are moved to nitrogen-limited medium, they undergo meiosis and produce haploid spores.
Komagataella pastoris has been successfully used for the production of a number of heterologous proteins. It can be used for both large-scale and small-scale production of proteins. Even though glycosylation in P. pastoris poses a big hurdle to the use of the expressed proteins for therapeutic purposes, glycoproteins such as vaccines that do not require authentic glycosylation to trigger a protective immune response can be produced in this system.
It is also possible to use P. pastoris to produce non-glycosylated proteins at high levels. Secretion of these proteins into the culture supernatant would make protein purification much simpler. In vivo isotope labelling of proteins for nuclear magnetic resonance studies is possible in this system.
Therefore P. pastoris serves as an excellent tool for the large-scale production of proteins for commercial purposes and also for the study of proteins. All these aspects make the Pichia expression system an attractive system for use by both industrial and academic research laboratories.
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