Môi trường NBRC 851

Medium Detail Information


Medium No.
Thermotoga maritima Medium
Soluble starch 5 g
KH2PO4 0.5 g
Trace minerals* 10 ml
NaCl 20 g
Bacto Yeast Extract (Difco) 0.5 g
Resazurin 1 mg
Na2S·9H2O** 0.5 g
Artificial sea water*** 250 ml
Distilled water 750 ml
Mix ingredients except Na2S·9H2O, adjust pH to 6.5 with NaOH, and autoclave under a N2 atmosphere. Separately autoclave and neutralize Na2S·9H2O as 5% solution under a N2 atmosphere. Anaerobically add Na2S·9H2O solution.
*Trace minerals
Nitrilotriacetic acid 1.5 g
MgSO4·7H2O 3 g
MnSO4·2H2O 0.5 g
NaCl 1 g
FeSO4·7H2O 0.1 g
CoSO4·7H2O 0.18 g
CaCl2·2H2O 0.1 g
ZnSO4·7H2O 0.18 g
CuSO4·5H2O 0.01 g
H3BO3 0.01 g
Na2MoO4·2H2O 0.01 g
NiCl2·6H2O 0.025 g
Na2SeO3·5H2O 0.8 mg
Na2WO4 0.8 mg
KAl(SO4)2·12H2O 4 mg
Distilled water 1 L
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
**The sulfide is prepared in a vessel sealed with a butyl rubber stopper. Autoclave under a N2 atmosphere. After cooling, the pH is adjusted to about 7.3 by adding of sterile 4-0.2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow color indicate the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.
***Artificial sea water
NaCl 27.7 g
MgSO4·7H2O 7 g
MgCl2·6H2O 5.5 g
CaCl2·2H2O 2.25 g
KCl 0.65 g
NaBr 0.1 g
H3BO3 30 mg
SrCl2·6H2O 15 mg
Citric acid 10 mg
KI 0.05 mg
Distilled water 1 L