Môi trường NBRC 851

Môi trường NBRC 851
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Medium Detail Information

 

Medium No.
851
Medium
Thermotoga maritima Medium
Composition
Soluble starch5g
KH2PO40.5g
Trace minerals*10ml
NaCl20g
Bacto Yeast Extract (Difco)0.5g
Resazurin1mg
Na2S·9H2O**0.5g
Artificial sea water***250ml
Distilled water750ml
Mix ingredients except Na2S·9H2O, adjust pH to 6.5 with NaOH, and autoclave under a N2 atmosphere. Separately autoclave and neutralize Na2S·9H2O as 5% solution under a N2 atmosphere. Anaerobically add Na2S·9H2O solution.
*Trace minerals
Nitrilotriacetic acid1.5g
MgSO4·7H2O3g
MnSO4·2H2O0.5g
NaCl1g
FeSO4·7H2O0.1g
CoSO4·7H2O0.18g
CaCl2·2H2O0.1g
ZnSO4·7H2O0.18g
CuSO4·5H2O0.01g
H3BO30.01g
Na2MoO4·2H2O0.01g
NiCl2·6H2O0.025g
Na2SeO3·5H2O0.8mg
Na2WO40.8mg
KAl(SO4)2·12H2O4mg
Distilled water1L
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
**The sulfide is prepared in a vessel sealed with a butyl rubber stopper. Autoclave under a N2 atmosphere. After cooling, the pH is adjusted to about 7.3 by adding of sterile 4-0.2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow color indicate the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.
***Artificial sea water
NaCl27.7g
MgSO4·7H2O7g
MgCl2·6H2O5.5g
CaCl2·2H2O2.25g
KCl0.65g
NaBr0.1g
H3BO330mg
SrCl2·6H2O15mg
Citric acid10mg
KI0.05mg
Distilled water1L
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