Môi trường JCM 14

Môi trường JCM 14
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Medium data

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14   EG MEDIUM

`Lab-Lemco’ powder (Oxoid) 2.4    g
Proteose peptone No. 3 (BD-Difco) 10.0    g
Yeast extract (BD-Difco) 5.0    g
Na2HPO4 4.0    g
Glucose 1.5    g
Soluble starch 0.5    g
L-Cystine 0.2    g
Agar 15.0    g
L-Cysteine·HCl·H2O 0.5    g
Horse blood 50.0    ml
Distilled water 1.0    L

Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6 – 7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.